Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• Publications: View 27 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 605
• Brilliant Violet 711
• eFluor 450
• eFluor 570
• eFluor 660
• FITC
• NovaFluor Red 710
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The II/41 monoclonal antibody reacts with the µ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly during early stages of B lymphopoiesis and on the surface of mature B cells in bone marrow and peripheral tissues. Fluorochrome-conjugated II/41 can be used as a detection secondary for mouse IgM.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤ 0.5 µg per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Titrate for optimal performance.

Fluorochrome Information:
PerCP-eFluor™ 710 can substitute for PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.

• Excitation: 488 nm (Blue Laser)
• Emission: 710 nm
  Recommended filters: 685 LP dichroic mirror and 710/40 band pass filter (695/40 acceptable alternative).
  Stable when exposed to 2% formaldehyde overnight at 4°C (evaluate for alternative fixation).

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgM is an immunoglobulin M antibody produced by B cells in response to antigens. It is the first antibody generated during immune response and the largest immunoglobulin in serum. Composed of five heavy and five light chains, it plays a key role in complement activation and antigen agglutination. Widely used in ELISA, Western blot, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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