Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), PerCP-eFluor 710, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Tested Dilution: 0.06 µg/test
• Publications: View 57 publications

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40, 17-1A antigen, TACSTD1, and CD326.
Generated using the TE-71 thymic epithelial cell line, CD326 is expressed on most epithelial cells and functions as a pan-carcinoma antigen mediating calcium-independent, homophilic cell-cell adhesion. It may also act as a growth factor receptor and is a target for immunotherapy in human carcinomas. CD326 binds LAIR-1 (CD305) and LAIR-2 (CD306), modulating cellular activation and inflammation.

• Reported Application: Flow cytometric analysis
• Tested Application: Flow cytometric analysis of TE-71 cell line
• Recommended Usage: ≤ 0.06 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
• Fluorochrome: PerCP-eFluor 710 (excitation 488 nm, emission 710 nm, Blue Laser)
• Recommended Filters: 685 LP dichroic mirror and 710/40 band pass (695/40 acceptable)
• Fixation Stability: Stable after 2% formaldehyde fixation overnight at 4°C
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Ep-CAM (Epithelial Cell Adhesion Molecule) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It mediates Ca²⁺-independent, homotypic cell adhesion and influences cell cycle, proliferation, and metabolism by inducing c-myc and cyclins A/E.
Ep-CAM overexpression is associated with enhanced epithelial proliferation and is a marker for tumors of epithelial origin. It is localized on the basolateral surface of most simple epithelia and many carcinoma types and is useful in distinguishing adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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