Thermo Fisher Scientific NMNAT1 Monoclonal Antibody (OTI1G4), TrueMAB
인간 NMNAT1 단백질을 인식하는 mouse IgG1 단일클론 항체로, Western blot, IHC, Flow cytometry에 사용 가능. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용. NAD 생합성 관련 연구에 적합한 TrueMAB 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G4 |
| Immunogen | Full length human recombinant NMNAT1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The coenzyme NAD and its derivatives are involved in numerous metabolic redox reactions and play roles in protein ADP-ribosylation, histone deacetylation, and Ca(2+) signaling pathways.
NMNAT (EC 2.7.7.1) is a key enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD.
(Zhang et al., 2003 [PubMed 12574164]; supplied by OMIM)
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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