Thermo Fisher Scientific ATG14 Recombinant Rabbit Monoclonal Antibody (10H20L14)
ATG14 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 면역세포염색에 적합. 높은 특이성과 민감도를 제공하며, 로트 간 일관성이 우수함. 인간에 반응하며 원숭이, 마우스 등에도 예측 반응. 단백질 A 정제, PBS buffer에 보존.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 10H20L14 |
| Immunogen | Multiple peptides corresponding to Human ATG14 (aa 63–79, 162–178, 277–293) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532500 |
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Mouse, Pig, Bovine, Rabbit, and Dog.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed to ensure high specificity and sensitivity.
Advantages include:
- Lot-to-lot consistency
- Animal origin-free formulation
- Enhanced immunoreactivity due to broader rabbit immune repertoire
Target Information
ATG14 (Autophagy related protein homolog 14) is encoded by the ATG14 gene on human chromosome 14. Also known as ATG14L or BARKOR (Beclin 1-associated autophagy-related key regulator), it plays a key role in autophagy, the cellular process for recycling misfolded proteins and degrading dysfunctional organelles.
Autophagy is induced under nutrient stress and involves formation of autophagosomes, which fuse with lysosomes to form autophagolysosomes.
The Class III PI3-Kinase Vps34 and Beclin 1 form a complex with ATG14 (type 1) or UVRAG (type 2). ATG14 directs the type 1 complex to the preautophagosomal structure (PAS), critical for autophagosome initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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