Thermo Fisher Scientific SMAD4 Recombinant Rabbit Monoclonal Antibody (10H10L15)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody is predicted to react with Monkey, Cat, Horse, Pig, Bovine, Rabbit, and Goat.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.
Advantages include:

• High specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broad immunoreactivity due to the rabbit immune repertoire

Target Information

Smad4 is encoded by the SMAD4 gene located on chromosome 18 in humans. It is also known as Smad family member 4, Sma- and Mad-related protein 4, or Mothers against decapentaplegic (MAD) homolog 4.
Smad4 contains N-terminal MH1 and C-terminal MH2 domains involved in DNA binding and protein interactions. Upon TGF-β or BMP ligand binding, receptor Smads (R-Smads: Smad1, 2, 3, 5, 8) are phosphorylated and form heterotrimers with Smad4 (co-Smad), which translocate to the nucleus to regulate gene expression.
Smad4 functions as a tumor suppressor by regulating target genes such as Cyclin D1 (downregulation) and collagen (upregulation), influencing cell proliferation and differentiation. Dephosphorylation controls the nucleocytoplasmic dynamics of Smads.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.