Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), Biotin, eBioscience

Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), Biotin, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1 µg/test
Publications: View 3 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 585 / 610-70S / 660-120S / 690
• NovaFluor Red 710 / 755
• NovaFluor Yellow 570 / 660 / 730 / 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

The 28-14-8 monoclonal antibody reacts with mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q.
Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes; ≤1 µg per test (100 µL final volume).
Cell number should be determined empirically (10^5–10^8 cells/test).
Careful titration recommended for optimal performance.

Target Information

This protein is an MHC class I alloantigen involved in antigen presentation to CD3/TCR and CD8-expressing T cells.
HLA and MHC antibodies are essential in Immunopeptidomics, aiding neoantigen identification and characterization via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.