Thermo Fisher Scientific TAP Tag Monoclonal Antibody (22F12-1E3), DyLight 680
TAP 태그 단백질 검출용 DyLight 680 결합 단클론 항체. Western blot에서 높은 특이성과 감도를 제공. 676/705 nm 파장에서 형광 검출 가능. TEV 절단 후 TAP C-말단을 인식. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 22F12-1E3 |
| Immunogen | KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage |
| Conjugate | DyLight™ 680 |
| Excitation / Emission Max | 676 / 705 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536817 |
Additional Formats
- Unconjugated (MA1-108)
- Biotin (MA1-108-BTIN)
- DyLight 488 (MA1-108-D488)
- DyLight 800 4X PEG (MA1-108-D800)
- HRP (MA1-108-HRP)
- Custom conjugation available upon request
Product Specific Information
MA1108-D680 detects the TEV cleavage peptide sequence.
This antibody has been successfully used for fluorescent detection of TAP in Western blot assays.
DyLight 680 has an excitation/emission of 692/712 nm.
Target Information
Epitope tags allow researchers to localize gene products, study protein topology and complexes, identify associated proteins, and characterize low abundance or poorly immunogenic proteins when protein-specific antibodies are unavailable.
Tandem affinity purification (TAP) is used to isolate TAP-tagged proteins along with their associated partners. The TAP tag typically includes:
- Calmodulin binding peptide (CBP)
- TEV protease cleavage site
- Protein A domain
This technique is valuable for analyzing in vivo protein interactions.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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