Thermo Fisher Scientific TAP Tag Monoclonal Antibody (22F12-1E3), DyLight 800 4X PEG
TAP 태그 단백질 검출용 모노클로날 항체로 DyLight 800 4X PEG 형광 표지. Western blot에 최적화되어 높은 특이성과 감도를 제공. TEV 절단 후 TAP C-말단 펩타이드 인식. 연구용으로만 사용 가능.
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Applications
- Western Blot (WB): Tested dilution 1:500–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 22F12-1E3 |
| Immunogen | KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage |
| Conjugate | DyLight™ 800 4X PEG |
| Excitation / Emission Max | 789 / 794 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | MES with proprietary stabilizer |
| Contains | No preservative |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536818 |
Additional Formats
- Unconjugated
- Biotin
- DyLight 488
- HRP
- Custom conjugation available upon request
Product Specific Information
MA1-108-D800 detects the TEV cleavage peptide sequence and has been successfully used for fluorescent detection of TAP in Western blot.
DyLight 800 4X PEG has an excitation/emission of 783/797 nm.
Target Information
Epitope tags enable localization of gene products in various cell types, study of protein topology and complexes, identification of associated proteins, and characterization of newly identified or low-abundance proteins when protein-specific antibodies are unavailable.
Tandem affinity purification (TAP) is an affinity purification method for isolating TAP-tagged proteins along with associated proteins. The TAP tag typically includes a calmodulin-binding peptide (CBP), a tobacco etch virus protease (TEV) cleavage site, and Protein A. This technique is valuable for analyzing in vivo interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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