Thermo Fisher Scientific CD137 Polyclonal Antibody, Biotin, PeproTech
인간 CD137(4-1BB) 수용체를 인식하는 염소 유래 Biotin 결합 폴리클로날 항체. Western blot 및 ELISA에 최적화되어 있으며 항원 친화 크로마토그래피로 정제됨. 활성 T세포 표지 및 면역반응 연구용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human 4-1BB Receptor |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
AA Sequence of recombinant protein:
MERTRSLQDP CSNCPAGTFC DNNRNQICSP CPPNSFSSAG GQRTCDICRQ CKGVFRTRKE CSSTSNAECD CTPGFHCLGA GCSMCEQDCK QGQELTKKGC KNCCFGTFND QKRGICRPWT NCSLDGKSVL VNGTKERDVV CGPSPADLSP GASSVTPPAP AREPGHS.
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human 4-1BB Receptor. Anti-Human 4-1BB Receptor-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect h4-1BB Receptor by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human 4-1BB Receptor (500-P167G) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant h4-1BB Receptor.
Western Blot:
To detect h4-1BB Receptor by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant h4-1BB Receptor is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Package Information:
500-P167GBT-1MG will be provided as 2 × 500 µg.
Target Information
CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells.
CD137 signaling maintains the survival of activated and CD8+ memory T cells, promotes clonal expansion of T cells, and modulates myelopoiesis and dendritic cell development. Triggered CD137 induces cytokine release that regulates peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanisms for immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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