Thermo Fisher Scientific p53 Monoclonal Antibody (Y5)

Thermo Fisher Scientific p53 Monoclonal Antibody (Y5)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA5-14467 targets p53 in IHC (P) applications and shows reactivity with human samples.
The immunogen is a synthetic peptide derived from N-terminal residues of human p53.

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides.
p53 up-regulates stress response genes such as p21waf1, leading to G1 or G2/M arrest. Structurally, p53 consists of:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerisation domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is critical for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation.
p53 plays a key role in blocking proliferation of damaged cells and acts as an anticancer agent. It is also linked to aging, oxidative stress, and cognitive functions such as long-term potentiation (LTP).
In normal cells, p53 is expressed at low levels, but in transformed cell lines, it is highly expressed and contributes to malignancy.
Mutations in the TP53 gene lead to loss of tumor suppressor activity and are found in many human cancers, including hereditary cancer syndromes such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.