Thermo Fisher Scientific WAVE1 Polyclonal Antibody
WAVE1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB, IHC, ICC/IF 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 액상 형태로 항원 친화 크로마토그래피로 정제되었으며, 높은 순도(>95%)를 보유.
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Thermo Fisher Scientific WAVE1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 88–132 of Human WAVE1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2718922 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. Purity is >95% as determined by SDS-PAGE.
Target Information
WASP (Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 involved in actin polymerization and cytoskeletal organization. The WASP family also includes VASP and Mena, which localize to focal adhesions and regulate actin cytoskeleton dynamics.
WAVE proteins, including WAVE1 and WAVE2, function similarly, mediating actin reorganization downstream of Rho family GTPases. WAVE1 and WAVE2 regulate membrane ruffling by inducing actin filament cluster formation in response to Rac activation.
These proteins cooperate with the Arp2/3 complex to promote actin filament formation. WAVE1 (also known as SCAR) is primarily expressed in the brain, while WAVE2 is broadly expressed, with high levels in peripheral blood leukocytes. WAVE3 forms a multiprotein complex linking receptor kinases and actin via a C-terminal verprolin homology domain. This complex transduces signals that modulate cell shape, motility, and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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