Thermo Fisher Scientific RPH3A Polyclonal Antibody
RPH3A 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며, 고순도(>95%) 보장. 연구용으로 신경내분비 세포의 Rab3A 관련 단백질 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 200–250 of Human RPH3A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2718920 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as confirmed by SDS-PAGE.
Target Information
Rabphilin-3A (RPH3A) contains two C2 domains (A and B) at the carboxy-terminal and a cysteine-rich region with two zinc finger motifs at the amino-terminal (residues 45–170). It belongs to the C-type tandem C2 protein family, including synaptotagmins and Doc2. Rabphilin-3A is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. It binds Rab3a/GTP/Mg²⁺ within residues 45–170 and interacts with calcium ions and phosphatidylinositol 4,5-bisphosphate-containing lipid vesicles through its C2 domains. Rabphilin-3A acts as a positive regulator of calcium-dependent exocytosis, while Rab3A serves as a negative regulator. Despite their association, they influence exocytosis independently, likely through interactions with an unidentified factor recognizing the Rab3 binding domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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