Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 488
DyLight 488 형광이 결합된 염소 유래 항-토끼 IgG(H+L) 2차 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 면역분석에 사용됩니다. 높은 특이성과 민감도를 제공하며, 항원 친화 크로마토그래피로 정제되었습니다. 4°C에서 보관하며, 연구용으로만 사용됩니다.
- 카탈로그번호
- 35552
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 1:25–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Rabbit IgG, whole molecule |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C (protected from light) |
| Shipping Conditions | Ambient |
| RRID | AB_844398 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #35552 has been successfully used in Western blot, IF, ICC, IHC, IP, and FACS applications.
This antibody reacts with the heavy chains of rabbit IgG and the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins.
Cross-reactivity with immunoglobulins from other species may occur.
Store protected from light at 4°C. To extend shelf life, add an equal volume of glycerol to reach ~50% glycerol and store at -20°C.
DyLight 488 Amax = 493 nm; Emax = 518 nm. Mole Dye/Mole Protein Ratio is lot-dependent.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins.
They are useful for detection, sorting, or purification of rabbit IgG and provide increased versatility through multiple detection systems (HRP, AP, fluorescence).
Signal amplification is achieved as multiple secondary antibodies bind to a single primary antibody.
These antibodies are generated by immunizing the host with pooled rabbit immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
Additional Conjugate Formats Available
- Alexa Fluor 488 (10 nm, 5 nm colloidal gold)
- AP, Biotin, DyLight 350 / 405 / 550 / 594 / 633 / 650 / 680 / 800 4X PEG
- FITC, HRP, PE-Cyanine5.5, TRITC, WesternDot 625
- Custom conjugation available upon request
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