
Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 405
DyLight 405 형광을 결합한 염소 유래 항-토끼 IgG(H+L) 2차 항체로, WB, IHC, ICC, Flow 등 다양한 면역실험에 사용 가능. 높은 특이성과 감도를 제공하며, 400/423 nm의 발광 특성을 가짐. 항원 친화 크로마토그래피로 정제되어 안정적이고 재현성 높은 결과를 보장.
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Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 405
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 4 µg/mL |
| Flow Cytometry (Flow) | 1:25–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Rabbit IgG, whole molecule |
| Conjugate | DyLight™ 405 |
| Excitation/Emission Max | 400/423 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1307537 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #35551 has been successfully used in Western blot, IF, ICC, IHC, IP, and FACS applications.
This antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. Cross-reactivity with immunoglobulins from other species may occur.
Store protected from light at 4°C until opened. To extend shelf life, add an equal volume of glycerol (final concentration ~50%) and store at -20°C.
DyLight 405: Amax = 400 nm; Emax = 420 nm. Mole Dye/Mole Protein Ratio is lot-dependent.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of rabbit antibodies.
Secondary antibodies provide versatility and enhanced sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, they are produced by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for high specificity and performance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Formats
- Alexa Fluor 488-10 nm colloidal gold
- Alexa Fluor 488-5 nm colloidal gold
- AP
- Biotin
- DyLight 350
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 633
- DyLight 650
- DyLight 680
- DyLight 800 4X PEG
- FITC
- HRP
- PE-Cyanine5.5
- TRITC
- WesternDot 625
- Custom conjugation available upon request
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