
Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 594
DyLight 594 형광 표지를 가진 염소 유래 항-토끼 IgG(H+L) 2차 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합. 고순도 항원 친화 크로마토그래피 정제, 높은 특이성과 감도 제공. 연구용 전용 제품.
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Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 594
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:2,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 1:25–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Rabbit IgG, whole molecule |
| Conjugate | DyLight™ 594 |
| Excitation/Emission Max | 587/614 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient |
| RRID | AB_1185570 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #35560 has been successfully used in Western blot, IF, ICC, IHC, IP, and FACS applications.
This antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. It may cross-react with immunoglobulins from other species.
Store protected from light at 4°C until opened. To extend shelf-life, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at −20°C.
DyLight 594 Amax = 593 nm; Emax = 618 nm. Mole Dye/Mole Protein Ratio is lot-dependent.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of rabbit targets.
Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, they are generated by immunizing the host with pooled rabbit immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats Available
- Alexa Fluor 488 (10 nm & 5 nm colloidal gold)
- AP
- Biotin
- DyLight 350, 405, 488, 550, 633, 650, 680, 800 4X PEG
- FITC
- HRP
- PE-Cyanine5.5
- TRITC
- WesternDot 625
- Custom conjugation available
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