Thermo Fisher Scientific SOX9 Monoclonal Antibody (GMPR9), eBioscience
SOX9 단백질을 인식하는 GMPR9 클론의 마우스 단일클론 항체로, 인간·마우스·랫트 시료에 반응합니다. Western blot, IHC, ICC, ChIP 등 다양한 응용에 적합하며, 액상 형태로 4°C 보관합니다. 크로마토그래피로 정제된 고순도 연구용 항체입니다.
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Thermo Fisher Scientific SOX9 Monoclonal Antibody (GMPR9), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | - |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | 1 publication |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GMPR9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865429 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
This GMPR9 monoclonal antibody reacts with human and mouse Sox9, a member of the Sry HMG-box (Sox) gene family of transcription factors. Sox9 contains a high mobility group (HMG-box) DNA-binding domain that recognizes the sequence AGAACAATGG. When bound to co-factors, Sox9 bends DNA, facilitating chromatin remodeling and transcriptional regulation.
Sox9 is expressed in multiple tissues during development and plays key roles in:
- Cartilage formation
- Sex determination (testes formation)
- Neural crest formation
- Retinal development
It regulates genes such as sox5, sox6, sox10, collagen type 2 alpha, collagen 9A1, CEACAM1, and snail2. Dysregulation of Sox9 is associated with craniofacial defects, cleft palate, sex reversal, and cancers (melanoma, lung adenocarcinoma, breast cancer). Sox9 is also expressed in adult stem cells of the liver, pancreas, and intestine.
Applications Reported:
Western blotting, IHC (formalin-fixed paraffin embedded sections), microscopy, and immunocytochemistry.
Applications Tested:
- IHC: ≤1 µg/mL (high or low pH antigen retrieval)
- ICC: ≤1 µg/mL (fixed and permeabilized human cells)
Careful titration recommended for optimal performance.
Target Information
SOX9 plays a role in sex determination and Sertoli cell differentiation. It is involved in chondrogenesis and regulates genes responsible for this process. Translocation of SOX9 can cause campomelic dysplasia. It contributes to testes formation, neuron-glia switching in spinal cord development, prostate epithelial growth, and neural crest precursor formation. SOX9 also regulates melanin production and is expressed in adult testis, heart, and fetal brain (confirmed by Northern blot analysis).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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