Thermo Fisher Scientific Angiogenin Monoclonal Antibody (26-2F), eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

20 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

ELISA (ELISA)

Assay-Dependent

-

Immunoprecipitation (IP)

Assay-Dependent

-

Neutralization (Neu)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

26-2F

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865425

Product Specific Information

Description: The monoclonal antibody 26-2F recognizes human angiogenin. Angiogenin is a secreted ribonuclease (RNASE)-family member and is important for the formation of new blood vessels as well as the stimulation of cell proliferation and survival. Cellular stress signals direct angiogenin to stress granules where angiogenin functions by hydrolyzing cellular tRNA resulting in modulation of protein translation and promotion of cell survival. Cytoplasmic angiogenin can bind to actin, forming complexes that activate protease cascades which can degrade laminin and fibronectin allowing endothelial cells to penetrate the basement membrane and migrate. In cancer cells, growth signals direct angiogenin translocation to the nucleus where binding of DNA promotor regions stimulates rRNA transcription resulting in a downstream increase in protein synthesis driving cell growth and survival. Angiogenin is upregulated in multiple cancers including breast, prostate, cervical, colorectal, lymphoma, and melanoma. During development and in adulthood, angiogenin is highly expressed in the spinal cord and contributes to motor neuron survival. Angiogenin is important for neuronal survival and is depleted in neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and Parkinson`s disease. This 26-2F antibody is reported to have both in vivo and in vitro neutralizing activity to angiogenin.

Applications Reported: This 26-2F antibody has been reported for use in cytokine neutralization, immunoprecipitation, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, ELISA, and immunocytochemistry.

Applications Tested: This 26-2F antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Angiogenin (ANG or ANG I) is important for the process of neovascularization and formation of new blood vessels. ANG is similar to pancreatic ribonuclease A and functions as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. It interacts with endothelial cell-surface actin and may cause changes in the cell cytoskeleton. ANG is thought to be involved in the development of solid tumors and its antagonists are capable of inhibiting tumor growth. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). Angiogenin is a genetic link between ALS and PD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.