Thermo Fisher Scientific Chicken anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, AP
닭 유래 항염소 IgG(H+L) 교차흡착 2차항체로 AP 효소 결합형입니다. Western blot, IHC, ELISA 등 다양한 면역분석에 적합하며, 높은 특이도와 신호 증폭 효과를 제공합니다. 친화 크로마토그래피 정제, 2.5 mg/mL 농도, 4°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 1% BSA, 30 mM triethanolamine, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534646 |
| Target | IgG |
| Cross Adsorption | Against human, mouse, and rabbit serum proteins |
| Antibody Form | Whole Antibody |
Available Conjugate Formats
- Alexa Fluor 488
- Alexa Fluor 594
- Alexa Fluor 647
- Biotin
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins. They are useful for detection, sorting, or purification of target antibodies. Secondary antibodies enhance versatility by enabling various detection systems (e.g., HRP, AP, fluorescence) and provide increased sensitivity through signal amplification, as multiple secondary antibodies can bind a single primary antibody. Typically, these antibodies are generated by immunizing the host with pooled immunoglobulins from the target species, followed by purification and optional modifications such as immunoaffinity chromatography, fragmentation, or conjugation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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