Thermo Fisher Scientific Chicken anti-Mouse IgG (H+L) Secondary Antibody, HRP

Applications

Tested Dilution

Western Blot (WB)

1:500-1:20,000

Immunohistochemistry (IHC)

1:500-1:20,000

ELISA (ELISA)

1:500-1:20,000

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

HRP HRP HRP

View additional formats

• [Biotin ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Biotin/host/Chicken/)
• Request custom conjugation

Form

Lyophilized

Concentration

2.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% ProClin 150

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2534649

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).

Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid. Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum immunoglobulins.

Target Information

Mouse IgG is an immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant immunoglobulin in the serum and plays a crucial role in the adaptive immune response. Mouse IgG is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. Mouse IgG is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.