Thermo Fisher Scientific Phospho-A-Raf (Thr214) Polyclonal Antibody
A-Raf 단백질의 Ser214 인산화 형태를 특이적으로 검출하는 rabbit polyclonal antibody. Western blot에 적합하며, Human, Mouse, Rat 시료에 반응. 1 mg/mL 농도의 액상 형태로, -20°C에서 보관. 연구용으로만 사용.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-A-Raf (Thr214) Polyclonal Antibody
Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ARAF (Accession P10398), corresponding to amino acid residues around phosphorylated Ser214 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816912 |
Product Specific Information
This antibody detects endogenous levels of A-RAF only when phosphorylated at Ser214.
Target Information
Several serine/threonine protein kinases function as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1.
Raf-1 is a cytoplasmic protein with intrinsic serine/threonine kinase activity and is the cellular homolog of v-Raf, the transforming gene product of the 3611 strain of murine sarcoma virus.
While v-Raf’s unregulated kinase activity is associated with transformation and mitogenesis, Raf-1 activity is normally suppressed by a regulatory N-terminal domain.
A-Raf, another member of the Raf gene family, shares substantial homology with Raf-1 within the kinase domain but less elsewhere.
A-Raf expression is highest in urogenital tissues and kidney, and lowest in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.