Thermo Fisher Scientific Phospho-A-Raf (Ser582) Polyclonal Antibody

Thermo Fisher Scientific Phospho-A-Raf (Ser582) Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:2,000

Species Reactivity

• Human
• Mouse
• Rat

Host / Isotype

• Rabbit / IgG

Class

• Polyclonal

Type

• Antibody

Immunogen

A synthesized peptide derived from human ARAF (Accession P10398), corresponding to amino acid residues around phosphorylated Ser582.

Conjugate

• Unconjugated

Form

• Liquid

Concentration

• 1 mg/mL

Purification

• Sequential chromatography

Storage Buffer

• PBS, pH 7.4, with 50% glycerol

Contains

• 0.02% sodium azide

Storage Conditions

• -20°C

Shipping Conditions

• Wet ice

RRID

• AB_2816911

Product Specific Information

This antibody detects endogenous levels of A-RAF only when phosphorylated at Ser582.

Target Information

Several serine/threonine protein kinases act as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1.
Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity, broadly expressed across cell lines, and is the cellular homolog of v-Raf, the transforming gene product of murine sarcoma virus strain 3611.
Unregulated kinase activity of v-Raf is associated with transformation and mitogenesis, whereas Raf-1 activity is normally suppressed by a regulatory N-terminal domain.
A-Raf, another member of the Raf gene family, shares substantial homology with Raf-1 in the kinase domain but less elsewhere. A-Raf expression is highest in urogenital tissues and kidney and lowest in brain tissue.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.