Thermo Fisher Scientific NBN Monoclonal Antibody (OTI3A4), TrueMAB
인간 NBN 단백질(aa 183-460)에 특이적인 Mouse monoclonal antibody. Western blot 및 IHC(P) 검증 완료. 동결건조 형태, PBS buffer(8% trehalose) 포함. 연구용으로 DNA 손상 복구 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 183–460 of human NBN produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The p95 gene (identical to NBS1 and nibrin) is part of the hMre11/hRad50 double-strand break complex.
This complex is implicated in Nijmegen breakage syndrome, an autosomal recessive disorder associated with increased cancer incidence, cell cycle checkpoint defects, and ionizing radiation sensitivity.
These findings reveal a molecular link between double-strand break repair and cell cycle checkpoint functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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