Thermo Fisher Scientific ALAS1 Recombinant Rabbit Monoclonal Antibody (SA70-04)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:5,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Advantages include:

• Improved specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broader immunoreactivity due to the larger rabbit immune repertoire

Target Information

ALAS1 is a nuclear-encoded mitochondrial matrix enzyme catalyzing the condensation of glycine with succinyl-CoA to form δ-aminolevulinate, CO₂, and CoA. It regulates the first and rate-limiting step of the heme biosynthetic pathway.
Key points:

• Ubiquitously expressed housekeeping enzyme dependent on pyridoxal-5′ phosphate
• Provides heme for cytochromes and hemoproteins
• Negatively regulated by heme in liver
• Expression stimulated by cAMP and respiratory uncoupling, repressed by phorbol esters and insulin
• Potential target for treatment of circadian disorders due to reciprocal regulation with heme biosynthesis
• Downregulated in acute liver failure, affecting heme metabolism and liver function

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일: MA5-31972_ALAS1_P13196-1_Rabbit.svg, MA5-31972_ALAS1_P13196-1_Rabbit_PDP.jpeg)