Thermo Fisher Scientific Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody (SA30-06)
Cytokeratin 19 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등에 적합합니다. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션으로 제조되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:5,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:500 | - |
| Flow Cytometry (Flow) | 1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SA30-06 |
| Immunogen | Synthetic peptide within Human Cytokeratin 19 aa 348–400 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809271 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Cytokeratin 19 is part of the intermediate filament protein family, showing biochemical diversity across human epithelial tissues. Human cytokeratins are numbered 1–20, varying in molecular weight (40–68 kDa) and isoelectric point (pH 4.9–7.8).
Cytokeratin expression patterns are characteristic of epithelial type and differentiation stage, aiding in the distinction of epithelial malignancies. Cytokeratin antibodies are valuable for tumor differential diagnosis, cytopathology, and flow cytometric assays. Cytokeratin 19 is not expressed in hepatocytes, making it useful for identifying liver metastases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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