Thermo Fisher Scientific BAD Recombinant Rabbit Monoclonal Antibody (SA32-01)
BAD 단백질을 인식하는 재조합 토끼 단일클론 항체로, 높은 특이성과 민감도를 제공합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. HEK293 발현 시스템에서 생산되며, 일관된 품질과 동물 유래 성분이 없는 포뮬레이션을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SA32-01 |
| Immunogen | Synthetic peptide within Human Bad (aa 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809272 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
Target Information
The protein encoded by BAD is a member of the BCL-2 family, known to regulate programmed cell death.
BAD positively regulates apoptosis by forming heterodimers with BCL-xL and BCL-2, reversing their death repressor activity.
Its proapoptotic activity is regulated through phosphorylation involving AKT, MAP kinase, and calcineurin.
Alternative splicing of the BAD gene produces two transcript variants encoding the same isoform.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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