Thermo Fisher Scientific GAPDH Recombinant Rabbit Monoclonal Antibody (SA30-01)
GAPDH 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry에 사용 가능. 높은 특이성과 재현성을 제공하며, Protein A 정제 및 안정한 저장 조건을 갖춤. 다양한 종(Human, Mouse, Rat, Zebrafish)에 반응.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:640,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SA30-01 |
| Immunogen | Recombinant protein within mouse GAPDH amino acids 94–333 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809270 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis and functions as a tetramer of 37-kDa subunits. Beyond glycolysis, GAPDH participates in DNA replication, repair, RNA export, membrane fusion, and microtubule bundling. It also plays roles in apoptosis, neurodegenerative diseases, and cancer-related processes. Due to its high and ubiquitous expression, GAPDH serves as a reliable protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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