Thermo Fisher Scientific Phospho-GSK3B (Ser9) Recombinant Rabbit Monoclonal Antibody (SY02-71)
Recombinant rabbit monoclonal antibody recognizing phospho-GSK3B (Ser9). Validated for WB, IHC, and ICC applications. Offers high specificity, sensitivity, and lot-to-lot consistency. Suitable for research use in signaling and metabolism studies.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY02-71 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser9 of Human GSK3 beta (aa 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809391 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal production candidates.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal development, and body pattern formation. It negatively regulates glucose homeostasis and participates in inflammation, ER stress, mitochondrial dysfunction, and apoptosis.
As a key component of the Wnt/β-catenin signaling pathway, GSK3B inhibits Wnt signaling by promoting β-catenin degradation. It is also implicated in Hedgehog, Notch, and insulin signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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