Thermo Fisher Scientific Phospho-HSF1 (Ser326) Recombinant Rabbit Monoclonal Antibody (SU31-03)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Specific antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the best candidates for production.
Advantages include:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broader immunoreactivity due to the large rabbit immune repertoire

Target Information

All organisms respond to elevated temperatures and various environmental stresses by synthesizing heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator heat shock factor (HSF), which binds to heat shock response elements (HSEs).
HSEs are composed of three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation upstream of all heat shock genes.
Although HSFs are conserved among species, their molecular weights differ and show limited immunological cross-reactivity.

In humans, two HSFs have been identified:

• HSF1: responsive to classical stress signals (heat, heavy metals, oxidative reagents)
• HSF2: activated during hemin-mediated differentiation of human erythroleukemia cells

HSF1 exists constitutively in the cytoplasm and nucleus as a monomer lacking DNA-binding activity. Upon stress, it becomes a nuclear-localized trimer that binds DNA. Phosphorylation of HSF1 is required for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.