Thermo Fisher Scientific CD20 Monoclonal Antibody (QCH6A7), Brilliant Violet 421, eBioscience
마우스 CD20을 인식하는 QCH6A7 클론 단일클론 항체로, Brilliant Violet 421 형광으로 표지됨. 유세포 분석용으로 최적화되어 높은 염색 강도 제공. B세포 표지 및 면역학 연구에 적합하며, 비특이적 결합 최소화를 위한 전용 버퍼 사용 권장.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD20 Monoclonal Antibody (QCH6A7), Brilliant Violet 421, eBioscience
Thermo Fisher Scientific CD20 Monoclonal Antibody (QCH6A7), Brilliant Violet 421, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | QCH6A7 |
| Conjugate | Brilliant Violet™ 421 |
| Excitation / Emission Max | 406 / 423 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2942141 |
Additional Formats
- APC
- Brilliant UV 661
- PE
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The monoclonal antibody QCH6A7 reacts with mouse CD20, a B cell-specific 32-kDa membrane protein expressed from late pro-B cells through memory B cells. CD20 is involved in intracellular calcium regulation and B cell proliferation. It is not found on hematopoietic stem cells or plasma cells.
CD20 expression begins after CD19 induction and increases during maturation. Unlike other B cell antigens, CD20 is not shed or internalized upon antibody binding. Monoclonal antibodies against CD20 are used to deplete B cells and are effective in B-cell lymphomas and autoimmune diseases such as rheumatoid arthritis and type I diabetes.
Clone QCH6A7 provides improved staining intensity compared to clone AISB12 in flow cytometric assays.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes and bone marrow cells at ≤1.0 µg/test (100 µL final volume).
Brilliant Violet™ 421 (BV421) emits at 423 nm and is suitable for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility.
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and malignant B cells, some T lymphocytes, and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 remains on the cell surface after antibody binding and serves as a therapeutic target for B-cell depletion.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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