Thermo Fisher Scientific PODN Polyclonal Antibody
Thermo Fisher Scientific의 PODN Polyclonal Antibody는 마우스 Podocan 단백질을 인식하는 염소 유래 다클론 항체입니다. Western blot 및 동결 절편 면역조직화학에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS 및 트레할로스 완충액에 동결건조 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–15 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Podocan (Val24–Arg611) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607082 |
Product Specific Information
- In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human Podocan is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Podocan (PODN, also known as PCAN or SLRR5A) is a non-collagenous protein belonging to the small leucine-rich proteoglycan (SLRP) family, class V subfamily.
It is expressed in kidney, heart, liver, pancreas, and vascular smooth muscle cells, and exists as three alternatively spliced isoforms containing twenty LRR (leucine-rich repeats).
Podocan contains a unique N-terminal cysteine-rich cluster and a highly acidic C-terminal domain.
It is a secreted glycoprotein with N-linked oligosaccharides and may play a role in growth regulation in cardiovascular tissues.
The gene encoding Podocan is located on human chromosome 1 and interacts with type I collagen (COL1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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