Thermo Fisher Scientific A33 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5-15 µg/mL

-

Flow Cytometry (Flow)

2.5 µg per million cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human A33 Ile22-Val235 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:A33,uniProtId:Q99795-1,ncbiNodeId:9606,antigenRange:22-235,antigenLength:319,antigenImageFileName:PA5-47444_A33_Q99795-1_Goat.svg,antigenImageFileNamePDP:PA5-47444_A33_Q99795-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2605829

Product Specific Information

In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse A33 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.