Thermo Fisher Scientific HIV1 nef Monoclonal Antibody (3D12)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

0.1-0.2 µg/mL

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Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3D12

Immunogen

Recombinant Nef protein of HIV-1 HXB2 purified from E. coli

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_962113

Product Specific Information

This antibody reacts with HIV-1 subtypes B and C, BRU, IIIB, and HAN strains. It has been mapped to amino acids 35-50 (RDLEKHGAITSSNTAA - HXB2).

This clone may also be referred to as 3D12 (01-002).

Target Information

HIV-1 Nef is one of the accessory proteins synthesized in the early stage of AIDS virus reproduction and is abundantly found in infected cells. The name derives from its negative factor thought at the beginning but presently it is remarked as the protein which bears a most distinctive biological characteristic of AIDS virus. The protein interacts directly with the signal transduction protein of the host T cell and works effectively on AIDS infection or on long term survival of the infected cells or induces apoptosis of non-infected cells. It is also involved in the endocytosis and degradation of receptor protein of the cell surface such as CD 4 and MH4, important for AIDS virus infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.