Thermo Fisher Scientific Phospho-p53 (Ser46) Polyclonal Antibody
Human p53 단백질의 Ser46 인산화 형태를 인식하는 Rabbit Polyclonal Antibody. Western blot 및 Immunocytochemistry에 적합. 항원 친화 크로마토그래피로 정제된 액상 제품으로 안정적이며 장기 보관 시 -20°C 권장. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Phospho-p53 (Ser46) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Human p53 (phospho Ser46). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.07 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736401 |
Product Specific Information
- Positive Control: 293T, 293T (0.5 µM doxorubicin for 24 hr)
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nucleotide starvation. Upon stress, p53 activates transcription of genes such as p21waf1, leading to G1 or G2/M arrest.
p53 structure includes:
- N-terminal transactivation domain
- Central DNA-binding domain
- Oligomerisation domain
- C-terminal regulatory domain
Phosphorylation at Ser15 is crucial for activation and stabilization. Phosphorylation at Ser392 is associated with tumor formation. p53 is linked to aging, oxidative stress, and learning/memory deficits. Normally expressed at low levels, it is upregulated in transformed cells, contributing to malignancy. Mutations in TP53 cause loss of tumor suppressor activity and occur in many cancers, including familial Li-Fraumeni syndrome.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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