Thermo Fisher Scientific CD103/Integrin E7 Monoclonal Antibody (ITGAE, 2063)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

CD103 (ITGAE, Integrin alpha E) is expressed on mucosa-associated T lymphocytes, activated cells, and a subset of TGF-β1 cells.
It consists of a 150 kDa alpha chain and a 120 kDa beta chain, expressed by 0.5–2% of resting lymphocytes in peripheral blood and lymphoid organs.
CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes.
As part of the integrin family, CD103 forms heterodimeric integral membrane proteins composed of an alpha and beta chain.
In combination with beta 7 integrin, CD103 forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen.
CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL) and plays roles in adhesion and IEL activation.
Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.