Thermo Fisher Scientific Phospho-Calmodulin 1/2/3 (Thr80, Ser82) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CALM1(Accession P0DP23), corresponding to amino acid residues around phosphorylated Ser82. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calmodulin 1/2/3,uniProtId:P0DP23-1,ncbiNodeId:9606,antigenRange:79|81|82,antigenLength:149,antigenImageFileName:PA5-104932_Calmodulin_123_P0DP23-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104932_Calmodulin_123_P0DP23-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Calmodulin 1/2/3,uniProtId:P0DP25-1,ncbiNodeId:9606,antigenRange:79|81,antigenLength:149,antigenImageFileName:PA5-104932_Calmodulin_123_P0DP25-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104932_Calmodulin_123_P0DP25-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816405

Product Specific Information

Antibody detects endogenous levels of Calmodulin1/2/3 only when phosphorylated at Thr80 + Ser82, which site historically referenced as Thr79+Ser81.

Target Information

Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.