Thermo Fisher Scientific THRB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

ChIP assay (ChIP)

-

View 3 publications 3 publications

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rabbit

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C-I(62) F H L D H D D V N D Q S V S S A Q T F(81) of human TR beta-1, with an N-terminal added cysteine. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:THRB,uniProtId:P10828-1,ncbiNodeId:9606,antigenRange:62-81,antigenLength:461,antigenImageFileName:PA1-213A_THRB_P10828-1_Rabbit.svg,antigenImageFileNamePDP:PA1-213A_THRB_P10828-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2203030

Product Specific Information

PA1-213A detects thyroid hormone receptor (TR) beta-1 from human and rat tissues. This antibody does not detect TR alpha-1 or TRv alpha-2.

PA1-213A has been successfully used in Western blot, immunohistochemistry (paraffin), IF and gel shift procedures. By Western blot, this antibody detects a ~55 kDa protein representing recombinant human TRv beta-1 expressed in E. coli.

PA1-213A immunizing peptide corresponds to residues 62-81 of human TR beta-1, with an N-terminal added cysteine. Alternative numbering of this protein has this peptide corresponding to residues 67-86 of human TR beta-1. This sequence is completely conserved between human and rat TR beta-1.

Target Information

Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1. The corresponding gene for the thyroid hormone receptor beta-1 is NR1A2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.