Thermo Fisher Scientific TLR2 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended positive controls: Human spleen sections or human peripheral blood.
• Store product as a concentrated solution.
• Centrifuge briefly prior to opening the vial.

Target Information

TLR2 is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate kinases and transcription factors including PKC alpha/beta and NF-kappa-B.
TLR2 forms heterodimers with TLR1 or TLR6, enabling recognition of bacterial and mycoplasma lipoproteins. TLRs are conserved from Drosophila to humans, sharing structural and functional similarities.
TLR2 is abundantly expressed in peripheral blood leukocytes and mediates host responses to Gram-positive bacteria and yeast via NF-kappaB activation. It aids in recognizing pathogen-associated molecular patterns (PAMPs) and stimulates cytokine production necessary for effective immunity.
Ten human homologs of TLRs (TLR1–10) have been described. TLR2 is expressed in tonsils, microglia, lymph nodes, appendices, and activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2, followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells.
Expression of TLR2 is regulated by immune response modifiers: LPS, GM-CSF, IL-1, and IL-10 upregulate TLR2, whereas IL-4, IFN-gamma, and TNF downregulate its expression in monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.