Thermo Fisher Scientific IL-2 Monoclonal Antibody (MQ1-17H12), eBioscience
인간 IL-2에 반응하는 비중화성 Rat 모노클로날 항체로 ELISA 캡처 및 Flow Cytometry용으로 검증됨. 높은 순도(>90%)와 낮은 응집도(<10%)를 보유하며 다양한 형광 접합체 옵션 제공. 연구용으로만 사용.
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Thermo Fisher Scientific IL-2 Monoclonal Antibody (MQ1-17H12), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 2 publications |
| Flow Cytometry (Flow) | Assay-dependent | 21 publications |
| ELISA | 1–4 µg/mL | 2 publications |
| Miscellaneous (PubMed) | - | 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Cynomolgus monkey, Human, Mouse, Non-human primate, Rhesus monkey |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MQ1-17H12 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468408 |
Available Conjugated Formats
- Alexa Fluor 532
- APC
- Brilliant UV 395
- Brilliant UV 615
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 480
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and major immunoregulatory cytokine. It is a non-neutralizing antibody.
Applications Reported:
- Capture antibody for ELISA
- Intracellular staining for flow cytometry
Applications Tested:
Used as capture antibody in sandwich ELISA for human IL-2 detection with biotin anti-human IL-2 poly (13-7028) and recombinant IL-2 (14-8029) as standard.
Recommended ELISA capture concentration: 1–4 µg/mL.
Standard curve range: 1000 pg/mL – 8 pg/mL.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Interleukin 2 (IL-2) is an immunomodulatory cytokine crucial for proliferation of activated T cells and differentiation of B cells, NK cells, monocytes, and macrophages.
IL-2 signals via the heterotrimeric IL-2 receptor (IL-2R), whose gamma chain is shared with IL-4 and IL-7.
Monoallelic expression of IL-2 in mature thymocytes represents a unique regulatory mechanism.
Mouse models lacking IL-2 exhibit ulcerative colitis-like symptoms, indicating IL-2’s essential role in immune response regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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