Thermo Fisher Scientific IL-4 Monoclonal Antibody (MP4-25D2), eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 7 publications 7 publications

ELISA (ELISA)

-

View 1 publication 1 publication

Neutralization (Neu)

-

View 2 publications 2 publications

Functional Assay (Functional)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MP4-25D2

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Functional Grade
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468414

Product Specific Information

Description: The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported: MP4-25D2 has been reported for use in neutralizing IL-4 binding and biological activity. (Please use Functional Grade purified MP4-25D2, Product # 16-7048 , in functional assays.) Please refer to the supporting protocols for more information.

Applications Tested: The purified MP4-25D2 can be used for blocking of fluorochrome conjugated MP4-25D2 in intracellular flow cytometry experiments to serve as specificity control. This biotinylated format for this lot of MP4-25D2 has been tested by sandwich ELISA for detection of human IL-4 in combination with 8D4-8 and can be used at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.