Thermo Fisher Scientific IL-2 Monoclonal Antibody (JES6-1A12), eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 7 publications 7 publications

ELISA (ELISA)

-

View 24 publications 24 publications

Immunoprecipitation (IP)

Assay-Dependent

-

Neutralization (Neu)

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View 7 publications 7 publications

Functional Assay (Functional)

-

View 30 publications 30 publications

Blocking Assay (BLOCK)

-

View 1 publication 1 publication

Immunostaining (IS)

1-4 µg/mL

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Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

JES6-1A12

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Functional Grade
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468406

Product Specific Information

Description: The JES6-1A12 antibody reacts with mouse interleukin-2 (IL-2). The JES6-1A12 antibody is a neutralizing antibody. Mouse IL-2 (also known as TCGF) is a 17 kDa factor produced mainly by activated CD4+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK cells, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions, and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.

Applications Reported: The JES6-1A12 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-2 bioactivity, IP, and intracellular staining.

Applications Tested: The JES6-1A12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-2 (IL-2) in combination with the biotin JES6-5H4 (13-7021) antibody for detection and recombinant mouse IL-2 (14-8021) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.