Thermo Fisher Scientific CD99 Monoclonal Antibody (M026)
CD99 단백질 검출용 Mouse IgG1 단일클론 항체로, WB, ICC/IF, ELISA, IP 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 단백질 G 정제, PBS/BSA/glycerol buffer, -20°C 보관. 연구용 한정.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M026 |
| Immunogen | Clone M026 was generated from a proprietary antigen related to the extracellular region of human CD99 expressed in the MeWo melanoma cell line. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | ~0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942637 |
Product Specific Information
Clone M026 detects 30 and 32 kDa bands corresponding to the molecular mass of the short and long forms of CD99 on SDS-PAGE immunoblots of human A431, LNCaP, MDA-MB-231, A549, and MeWo cell lysates, as well as mouse LL2 lung carcinoma.
The antibody can be used for multiple applications including ELISA capture, western blot, immunocytochemistry, and immunoprecipitation.
In addition, the antibody labels live, unfixed MeWo cells.
Target Information
CD99 (E2, MIC2) is a transmembrane glycoprotein involved in regulation of T cell adhesive properties and programmed cell death distinct from typical apoptosis.
CD99 functions are specific to certain stages of T cell differentiation such as corticothymocytes.
CD99R isoform expression is restricted in the hematopoietic system to T, NK, and myeloid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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