Thermo Fisher Scientific CD99 Monoclonal Antibody (M026)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M026

Immunogen

Clone M026 was generated from a proprietary antigen related to the extracellular region of human CD99 expressed in the MeWo melanoma cell line.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942637

Product Specific Information

Clone M026 detects 30 and 32 kDa* bands corresponding to the molecular mass of the short and long forms of CD99 on SDS-PAGE immunoblots of human A431, LNCaP, MDA-MB-231, A549, and MeWo cell lysates, as well as mouse LL2 lung carcinoma. The antibody can be used for multiple applications including ELISA capture, western blot, immunocytochemistry, and immunoprecipitation. In addition, the antibody labels live, unfixed MeWo cells.

Target Information

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.