Thermo Fisher Scientific CD47 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25-1 µg/mL

-

Immunohistochemistry (IHC)

1-15 µg/mL

-

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD47 N-terminal IgV-like Gln19-Pro158 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD47,uniProtId:Q61735-1,ncbiNodeId:10090,antigenRange:19-158,antigenLength:303,antigenImageFileName:PA5-47215_CD47_Q61735-1_Goat.svg,antigenImageFileNamePDP:PA5-47215_CD47_Q61735-1_Goat_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2607373

Product Specific Information

In direct ELISAs, less than 15% cross-reactivity with recombinant human CD47 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.