Thermo Fisher Scientific PP2A alpha Polyclonal Antibody
Western blot 및 Immunoprecipitation에 사용 가능한 PP2A alpha 폴리클로날 항체. Rabbit IgG 기반으로 고순도 항원 친화 크로마토그래피 정제. 다양한 종에 예측 반응성을 보이며, 세포 신호 조절 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PP2A alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.56 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792789 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (100%), Bovine (100%).
- Positive Control: 293T, A431, HeLa, HepG2.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis, and apoptosis.
A group of proteins intimately involved in this process are the protein phosphatases.
The protein phosphatase (PP) holoenzyme is generally a trimeric complex composed of a regulatory subunit, a variable subunit, and a catalytic subunit.
Four major families of protein phosphatase catalytic subunits have been identified: PP1, PP2A, PP2B (calcineurin), and PP2C.
The PP2A family includes subfamily members PP2A alpha and PP2A beta. An additional catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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