Thermo Fisher Scientific Phospho-AMPK alpha-1,2 (Thr172, Thr183) Polyclonal Antibody
AMPK α1/α2의 Thr172 및 Thr183 인산화 형태를 인식하는 Rabbit Polyclonal 항체. Western blot에 적합하며, 다양한 종에 예측 반응성을 보임. 에너지 대사 연구 및 AMPK 신호전달 분석에 유용. 항원 친화 크로마토그래피로 정제된 고품질 항체.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human AMPK alpha 1 (phospho Thr183) and AMPK alpha 2 (phospho Thr172) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2792788 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Bovine (100%).
- Positive Control:
- MCF-7 (100 μM CCCP treatment for 2 hr)
- HeLa (500 μM CoCl₂ treatment for 22 hr and 10 μM MG132 treatment for 2 hr)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK).
AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits.
AMPK functions as an energy-sensing enzyme that monitors cellular energy status. In response to metabolic stress, AMPK is activated and phosphorylates key enzymes such as acetyl-CoA carboxylase (ACC) and HMG-CoA reductase (HMGCR), regulating fatty acid and cholesterol biosynthesis.
Studies in mice suggest that this catalytic subunit controls whole-body insulin sensitivity and is essential for maintaining myocardial energy homeostasis during ischemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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