Thermo Fisher Scientific Phospho-AMPK alpha-1,2 (Thr172, Thr183) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AMPK alpha-1,2,uniProtId:Q13131-1,ncbiNodeId:9606,antigenRange:183,antigenLength:559,antigenImageFileName:PA5-85649_AMPK_alpha-1,2_Q13131-1_Rabbit.svg,antigenImageFileNamePDP:PA5-85649_AMPK_alpha-1,2_Q13131-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:AMPK alpha-1,2,uniProtId:P54646-1,ncbiNodeId:9606,antigenRange:172,antigenLength:552,antigenImageFileName:PA5-85649_AMPK_alpha-1,2_P54646-1_Rabbit.svg,antigenImageFileNamePDP:PA5-85649_AMPK_alpha-1,2_P54646-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2792788

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Bovine (100%).

Positive Control: MCF-7(100uM CCCP treatment for 2 hr), HeLa(500 uM CoCl2 treatment for 22 hr and 10 uM MG132 treatment for 2 hr).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.