Thermo Fisher Scientific Phospho-Crk (Tyr251) Polyclonal Antibody

Applications and Tested Dilution

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-Crk (Tyr-251) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-251 in human Crk II. This peptide sequence is well conserved in mouse and rat Crk II, as well as in CrkL (Tyr-251). The site is not found in Crk I.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.75 mg/mL

Purification

Antigen affinity chromatography

Storage Buffer

PBS with 1 mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage Conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping Conditions

Wet ice

RRID

AB_2942901

Product Specific Information

The antibody detects 38 and 40 kDa proteins corresponding to the molecular mass of Crk II and CrkL on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate and A431 cells stimulated with EGF. This reactivity is not observed after alkaline phosphatase treatment.

Target Information

Crk-I and Crk-II differ in their biological activities. Crk-II has less transforming activity than Crk-I and mediates attachment-induced MAPK8 activation, membrane ruffling, and cell motility in a Rac-dependent manner. It is involved in phagocytosis of apoptotic cells and cell motility via interaction with DOCK1 and DOCK4 and may regulate EFNA5-EPHA3 signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.