Thermo Fisher Scientific Phospho-CRMP2 (Thr555) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-CRMP2 (Thr-555) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Thr-555 in human CRMP2. This sequence is conserved in rat and mouse CRMP2 and the phospho-site is not conserved in other CRMP family members.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942904

Product Specific Information

This antibody was cross-adsorbed to unphosphorylated CRMP2 (Thr-555) before affinity purification using phospho-CRMP2 (Thr-555) peptide (without carrier). The antibody detects a 70 kDa* protein corresponding to CRMP2 on immunoblots of rat PC12 or mouse C2C12 cells treated with calyculin A. This reactivity can be specifically blocked using phospho-CRMP2 (Thr-555) peptide (CX2255).

Target Information

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.