Thermo Fisher Scientific beta-NGF Monoclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

4.0-8.0 µg/mL

-

ELISA (ELISA)

2.0-4.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2942207

Product Specific Information

AA Sequence of recombinant protein: SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA.

Preparation: Produced in mice immunized with highly pure Recombinant Human beta-NGF. Anti-Human beta-NGF-specific antibody was purified from cell culture by Protein G affinity chromatography.

Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 200 pg/mL of Recombinant Human beta-NGF when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human beta-NGF (500-P85BT) as the detection antibody at a concentration of approximately 0.5- 1.0 µg/mL.

Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human beta-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.

Target Information

Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.