Thermo Fisher Scientific SOX2 Monoclonal Antibody (Btjce), eFluor 570, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• eFluor 660
• Request custom conjugation

Product Specific Information

The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y-related HMG Box) family of proteins. Sox2 is critical for regulating genes involved in mammalian embryogenesis and maintaining pluripotency in embryonic stem cells (ESCs). It is co-expressed with Oct4, Klf4, and c-Myc in the generation of induced pluripotent stem cells (iPSCs).

Sox2 expression is tightly regulated; small changes can trigger differentiation into multiple cell types. Beyond embryonic stem cells, Sox2 is essential for early neurogenesis and neural stem cell maintenance. Abnormal Sox2 expression has been linked to various cancers including colorectal and breast cancer.

Applications Reported: Microscopy, immunohistochemical staining, and immunocytochemistry.
Applications Tested: Tested in ICC on fixed and permeabilized F9 cells; recommended ≤5 µg/mL. Optimize concentration for each assay.

Filter Recommendation:
For eFluor® 570 conjugates, use filters capturing 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70). Standard Alexa Fluor® 555 or TRITC filters are also suitable.

Excitation/Emission: 555 nm / 570 nm
Epitope: Bacterial expression of region after aa135 (homology between species but not to other family members)

Target Information

SOX2 is an intronless gene encoding a transcription factor from the SRY-related HMG-box (SOX) family, regulating embryonic development and cell fate determination. The gene is essential for stem-cell maintenance in the central nervous system and regulation of gene expression in the stomach. Located within an intron of SOX2 overlapping transcript (SOX2OT), SOX2 can act as a transcriptional activator through protein complex formation. Mutations are associated with bilateral anophthalmia, optic nerve hypoplasia, and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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