Thermo Fisher Scientific Nestin Monoclonal Antibody (10C2), eFluor 570, eBioscience
인간 Nestin 단백질(1464-1614)을 인식하는 10C2 클론의 단일클론 항체로, eFluor 570 형광 표지. 줄기세포 및 신경세포 마커 연구에 적합하며, ICC/IHC 등 다양한 형광 이미징 응용 가능. 555/570 nm 파장에서 최적 성능.
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Thermo Fisher Scientific Nestin Monoclonal Antibody (10C2), eFluor 570
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 publications |
| Immunohistochemistry (IHC) | - | 1 publication |
| IHC (Paraffin) | Assay-Dependent | - |
| IHC (Frozen) | Assay-Dependent | 1 publication |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | 3 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10C2 |
| Conjugate | eFluor™ 570 |
| Excitation / Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573652 |
Additional Formats
Product Specific Information
Description:
The monoclonal antibody 10C2 recognizes human nestin (residues 1464–1614). Nestin is a 220–240 kDa Class VI intermediate filament protein expressed in stem cells of the developing nervous system. Expression is also found in pancreatic islet and mesenchymal precursors, as well as cells with angiogenic potential. Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins such as GFAP. Expression has been identified in various cancer cells including glioblastomas and pancreatic tumors. No cross-reactivity to rodent nestins has been observed.
Applications Reported:
Useful for immunohistochemical staining of frozen and paraffin-embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested:
Validated for immunocytochemistry of formaldehyde-fixed and permeabilized human cells. Recommended usage ≤ 1 µg/mL, with titration for optimal assay performance.
Filter Recommendation:
For eFluor® 570 conjugates, use filters capturing 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70). Alexa Fluor® 555 or TRITC filters are also acceptable.
Excitation / Emission:
Excitation 555 nm; Emission 570 nm
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and early embryonic neuroepithelial stem cells. It serves as a marker for stem/progenitor cells, glioma cells, and tumor endothelial cells, and is a superior angiogenic marker for evaluating tumor neovascularity. Highly specific antibodies to human Nestin aid in characterizing progenitor cell differentiation, enhancing therapeutic potential of neural stem/progenitor cells for CNS disease treatment, and identifying neuroepithelial tumor cells. Nestin expression is also seen in pancreatic hematopoietic stem cell populations and proliferating endothelial cells in colorectal cancer tissue.
Note:
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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