Thermo Fisher Scientific CD1d Monoclonal Antibody (WTH-2), APC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

WTH-2

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573136

Product Specific Information

Description: The monoclonal antibody WTH-2 reacts with mouse and rat CD1d. CD1d is a non-polymorphic MHC Class I-like molecule composed of alpha-1 and alpha-2 domains. CD1d is constitutively expressed on hematopoietic cells, with highest levels on dendritic cells, macrophages, and marginal zone B-cells. It is also expressed on non-lymphatic organs such as the liver, heart, small intestine, and pancreas. CD1d associates with beta2-microglobulin to present antigens containing a lipid or other hydrophobic moieties to invariant NKT cells, which leads to rapid Th1 or Th2 cytokine secretion.

The WTH-2 antibody has been reported to have antagonist activity.

Applications Reported: This WTH-2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This WTH-2 antibody has been tested by flow cytometric analysis of mouse or rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD1d is a heavy chain associated with Beta 2 microglobulin on cortical thymocytes. Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins. The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by the CD1d gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.