Thermo Fisher Scientific CD1d Monoclonal Antibody (WTH-2), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
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Product Specifications

Product Specific Information

Description:
The monoclonal antibody WTH-2 reacts with mouse and rat CD1d. CD1d is a non-polymorphic MHC Class I-like molecule composed of alpha-1 and alpha-2 domains. It is constitutively expressed on hematopoietic cells, with highest levels on dendritic cells, macrophages, and marginal zone B-cells. It is also expressed on non-lymphatic organs such as the liver, heart, small intestine, and pancreas. CD1d associates with beta2-microglobulin to present antigens containing a lipid or other hydrophobic moieties to invariant NKT cells, leading to rapid Th1 or Th2 cytokine secretion.

The WTH-2 antibody has been reported to have antagonist activity.

Applications Reported:
This WTH-2 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
This antibody has been tested by flow cytometric analysis of mouse or rat splenocytes and can be used at ≤0.25 µg per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test). Titrate for optimal performance.

• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD1d is a heavy chain associated with beta-2 microglobulin on cortical thymocytes. Beta-2 microglobulin–independent expression of CD1d has also been demonstrated on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of MHC-like glycoproteins. The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which form heterodimers with beta-2-microglobulin. These proteins mediate presentation of lipid and glycolipid antigens to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1, differing in localization and ligand specificity. The CD1d protein localizes to late endosomes and lysosomes via a tyrosine-based motif in its cytoplasmic tail. Diseases associated with CD1D include Mycobacterium malmoense infection and autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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